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KMID : 1033620150420020051
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Clinical and Experimental Reproductive Medicine
2015 Volume.42 No. 2 p.51 ~ p.57
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Effect of laser-assisted multi-point zona thinning on development and hatching of cleavage embryos in mice
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Lee Young-Seok
Park Min-Jung
Park Sea-Hee
Koo Ja-Seong
Moon Hwa-Sook
Joo Bo-Sun
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Abstract
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Objective: This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age.
Methods: Eight-cell or morula embryos collected from superovulated C57BL female mice with different ages (6-11 and 28-31 weeks) were treated with LAZT at one-point (LAZT1) or four-points (LAZT4). The zona pellucida was thinned to more than 70% of its initial thickness by making two holes of 15-20 μm.
Results: In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05). However, in the old mice, LAZT significantly increased blastocyst formation as well as early hatching and hatching compared to the controls (p<0.05). These effects were more remarkable in LAZT4 than in LAZT1 and in aged mice than in young ones.
Conclusion: These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.
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KEYWORD
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Blastocyst formation, Hatching, Laser-assisted zona thinning, Multipoint laser-assisted zona thinning
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